Red spinach is a favorite vegetable of the Indonesian people because it has a fairly high nutritional content. However, its productivity has decreased due to the reduction of soil nutrients and the use of chemical fertilizers (inorganic) by farmers, in addition to the type of soil that is generally used for planting in Sorong Regency, West Papua, namely the type of oxysol soil that has progressed. Weathering with high content is undoubtedly due to high rainfall. For this reason, this study was conducted to determine the growth response and yield as well as the best dose of bokashi fertilizer to optimize the growth and yield of red spinach (Amaranthus tricolor L.) in Oxisol soil in Sorong Regency. This study used a single factor with a randomized block design consisting of 4 levels of treatment. Each treatment was repeated 3 times to obtain 12 experimental trials. The dosage of bokashi fertilizer treatment is as follows: P0= bokashi fertilizer 20 g/polybag; P1= bokashi fertilizer 40 g/polybag; P2= bokashi fertilizer 60 g/polybag; and P = 80 g/polybag of bokashi fertilizer. The results showed that the application of bokashi fertilizer on Oxisol topsoil planting media significantly affected the growth and yield of red spinach and the nutrient content of Oxisol growing media also increased after harvest. Treatment of 80 gr bokashi fertilizer (P3) gave optimal results and the best production with a plant height of 51.5 cm, a number of leaves 13.3 strands, and a wet weight of 19.4 gr.