“Petroganik” fertilizer is organic fertilizer derived from chicken and cow manure processed through a manufacturing process and has a high C-organic and macronutrient content. So, the research was conducted by testing the various doses of petroganik fertilizer on the growth and yield of batik spinach (Amaranthus tricolor L) in Alfisol soil, Sorong Regency. This research was conducted using a Randomized Block Design (RBD) with the treatment of 4 doses of Petroganik fertilizer and repeated 3 times so that there were 12 experimental plots. The dose of petroganik fertilizer used in this study consisted of P0= without giving petroganik fertilizer (control); P1= application of 1 kg/m2 of petroganik fertilizer beds; P2= application of 2 kg/m2 of petroganik fertilizer beds; and P3= application of petroganik fertilizer to 3 kg/m2 beds. Each experimental plot was taken with as many as 10 batik spinach plants to be used as sample plants. So, the total sample plants used were 120 plants. Based on the results of the study, it is known that the application of Petroganik fertilizer to the soil can increase the fertility status of Alfisol soil and petroganik fertilizer has a very significant effect on the growth and yield of batik spinach (Amaranthus tricolor L.) plants. The dose of petroganik fertilizer of 2 kg/m2 beds resulted in better plant height, leaf number, leaf area, stem diameter, and wet weight of batik spinach plants than the treatment at a dose of 3 kg/m2 beds and a dose of 1 kg/m2 beds.