Abstract : This study was conducted to determines: (1) the influence of bottom heat on the regeneration of Eugenia stem cuttings; (2) the influence of types of cutting on the regeneration of Eugenia stem cuttings; (3) the influence of different levels of Naphthalene Acetic Acid (NAA) on the regeneration of Eugenia stem cuttings; and (4) the interaction effect of bottom heat treatment and the different levels of concentration of NAA on the regeneration of Eugenia stem cuttings. The experiment Three-Way Factorial design has three grouping factors were laid out in 2 x 3 x 6 factorials in split plots arranged to take the first factor as main and remaining two factors as sub-factors in RCBD with three replications. The main whole of B1- with bottom heat and B2- without bottom heat; sub-plot of C1-no axillary shoots, C2- single axillary shoots, and C3- two axillary shoots; and sub-sub plot of NAA with T1- 0 ppm, T2- 500 ppm, T3- 1000 ppm, T4- 1500 ppm, T5- 2000 ppm, and T6- 2400 ppm. Bottom heat application of significantly promoted shoot productions both in terms of numbers of shoot and size of leaves of successfully regenerated cuttings. Cuttings without axillary shoots retained more leaves and gave a higher rooting percentage than cuttings with 2 axillary shoots. Moreover, the application of NAA significantly enhanced leaf retention; increased rooting percentage; number, diameter, and length of roots; the fresh and dry weight of roots; and roots starch and sugar contents. It also significa